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Image Search Results
Journal: Scientific Reports
Article Title: 96 perfusable blood vessels to study vascular permeability in vitro
doi: 10.1038/s41598-017-14716-y
Figure Lengend Snippet: Media optimization on microvessels using the permeability assay. ( a ) At the start of the assay (t = 0 min), the fluorescent dextrans are contained within the microvessel. Scale bar: 200 µm. ( b ) After 30 min, the differences in permeability are clearly visible: the dextran fills the gel in case of a completely permeable microvessel, while an impermeable vessel retains the dextran within the lumen of the vessel. The dotted line indicates the position of the cell barrier. Scale bar: 200 µm. ( c ) Visualization of dextran diffusion after 30 minutes for an array of 86 microvessels with three different culture conditions (M199 medium = M199, MV2 medium = MV2, Pericyte medium = PC). Excluded vessels due improper barrier formation or gel seeding are indicated with a diagonal hatch pattern. Scale bar: 2 mm. ( d ) Quantification of the permeability over different days for M199 (n = 28), MV2 (n = 29) and PC (n = 29). Data is presented as mean ± SD.
Article Snippet: MV2 endothelial cell Growth Medium (Promocell, C-22022) and
Techniques: Permeability, Diffusion-based Assay